Hemoglobin immobilized on ionic liquid modified functionalized multi-walled carbon nanotubes

کد مقاله : 1143-17THECSI

نویسندگان

عبدالمجید بایندری مقدم * عضو هیات علمی

چکیده مقاله

The direct electron transfer (DET) between proteins and bare solid electrode is difficult because the electroactive centers are embedded deeply in the protein structure, as well as adsorptive denaturation and unfavorable orientations of proteins on electrode surfaces [1]. In present paper, we used the simple casting method to immobilize Hemoglobin (Hb) on the surface of carbon ionic liquid electrode (CILE), which was constructed of graphite powder and ionic liquid, 1-butyl pyridinium hexaflourophosphate ([BuPy][PF6]), achieved direct electrochemistry of Hb and investigated electrocatalytic properties of Hb [2]. For biosensor preparation, 5 mg of MWCNTs and 38 mg of BPPF6 were added into 2.5 ml of N, N-Dimethyl formamid (DMF) solution as the stock solution. After approximately 15 min of sonication, uniformly dispersed MWCNTs-BPPF6 were formed .Then 10 µL of the prepared suspension was dipped onto GCEs to obtain the MWCNTs-BPPF6/glassy carbon (GC)-modified electrodes. After being air-dried, the MWCNTs-BPPF6-modified electrodes were immersed into the aqueous solution of Hb (10 mg Hb/3 mL PBS) for 24 h. The electrodes were then rinsed thoroughly with distilled water to remove the nonadsorbed Hb. The modified electrodes were stored in PBS in a refrigerator when not in use. The process can be expressed as follows: Fe(III) + H2O2 Compound I + H2O Compound I + 2e- + 2H+ Fe(III) + H2O We have demonstrated that the hemoglobin was immobilized on the surface of CILE/GC electrode and the direct electron transfer of hemoglobin was assessed by cyclic voltammetry. Hb/CILE/GC electrode showed a great sensitivity for H2O2.

کلیدواژه ها

Hemoglobin, Biosensor, Bioelectrochemistry, Bioanalysis

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