| Ultrasensitive Detection of Acinetobacter Baumannii Bacteria Using a Target‑Induced Redox Signal Amplification |
| کد مقاله : 1103-17THECSI |
| نویسندگان |
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رخساره عابدی *1، جهانبخش رئوف1، مجتبی محسنی2، ائمه باقری حشکوائی3 1گروه شمی تجزیه، دانشکده شیمی دانشگاه مازندران، بابلسر، ایران 2گروه میکروبیولوژی، دانشکده علوم زیستی، پردیس دانشگاه مازندران، بابلسر، مازندرلن 3گروه مهندسی برق و کامپیوتر، دانشگاه ایالتی کارولینای شمالی، رالی، کارولینای شمالی 27606، ایالات متحده |
| چکیده مقاله |
| The current research has reported a selective electrochemical aptasensor for the ultrasensitive detection of Acinetobacter baumannii (A. baumannii), a bacterium responsible for a wide range of nosocomial infections. The proposed system was developed by modifying screen-printed carbon electrodes (SPCE) with an MWCNT@Fe3O4@SiO2-Cl nanocomposite and then using a covalent immobilization of an A. baumannii -specific aptamer (Apt) onto the modified electrode surface, and afterward interaction of methylene blue (MB) with the Apt as an electrochemical redox marker. Upon the incubation of the A. baumannii as a target on the proposed aptasensor, the peak current of MB decreased due to the formation of the Apt-A. baumannii complex and the release of MB from the immobilized Apt on the surface of modified electrode. The suggested aptasensor was demonstrated to be capable of detecting A. baumannii with a linear range of 10.0 – 1.0×107 colony-forming unit (CFU) mL−1 and a detection limit of 1 CFU mL−1 (S/N=3) using differential pulse voltammetry (DPV) technique. The outcomes revealed that the aptasensor exhibited high A. baumannii detection sensitivity, stability, reproducibility, and specificity. |
| کلیدواژه ها |
| Acinetobacter baumannii; Electrochemical aptasensor; Nanocomposite, Methylene blue |
| وضعیت: چکیده برای ارائه شفاهی پذیرفته شده است |