| Ultrasensitive electrochemical biosensor for detection of serum HER2 using nanometal as a signal amplification modifier |
| کد مقاله : 1046-17THECSI |
| نویسندگان |
|
فرزانه حسینی دخت *1، نفیسه صحرائی2 1دانشگاه یزد 2دانشکده شیمی، پردیس علوم، دانشگاه یزد، یزد، ایران |
| چکیده مقاله |
| The main breast cancer-related protein biomarkers for non-invasive clinical tests are the Human Epidermal growth factor Receptor 2 (HER2 or ErbB2), Cancer Antigen 15-3 (CA 15-3), and Carcinoembryonic Antigen (CEA) [1] HER2 stimulates the growth of breast cancer cells as a result the accurate detection of Human epidermal growth factor receptor-2 (HER2) as an important breast cancer biomarker can be essential for the early selection of therapeutic approaches [2].In the current study, a novel and highly selective aptasensor was developed on an electrode surface for the ultrasensitive detection of HER2 through an strategy of functionalized GCE with the Ni foam and (Ag and AuNPs) as an efficient nanocomposite. In the proposed sensing strategy, the aptamer was covalently attached on the Nano metals, which was in turn attached on the surface of a glassy carbon electrode (GCE) modified. By combining the advantages of each nanocomposites component like stability of Ni foam, excellent conductivity of AgNPs nanometal,the synthesized nanocomposite exhibited very well electrocatalytic activity in the electrode surface. Furthermore, the electrode response changes at each stage after the modification with the nanomaterials, aptamer and HER2 was efficiently investigated using EIS technique. Different concentrations of CEA were measured by aptasensor under optimized experimental conditions , and their current responses were recorded by the DPV method. the designed aptasensor showed a wide linear response range of 10-13-10-6 µg.mL−1, the low detection limit of 10 pg.mL−1, and good selectivity and excellent detection of CEA in serum samples |
| کلیدواژه ها |
| Aptasensor, HER2, Breast cancer, Ni Foam, Ag and Au nanoparticles |
| وضعیت: چکیده برای ارائه به صورت پوستر پذیرفته شده است |